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KMID : 0903519740170010049
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1974 Volume.17 No. 1 p.49 ~ p.53
Comparative Studies on Microbioassay and Chemical Procedure for Quantitative Determination of Niacin in Feed Additives



Abstract
Mohan¢¥s aniline procedure for the quantitative determination of niacin in feed additives containing various vitamines and minerals was compared with microbiological procedure utilizing growth rate of Lactobacillus arabinosus 17-5. Microbioassay was more sensitive than chemical procedure to the detection of standard niacin and was applicable to the determination of minute amounts of niacin. while both microbioassay and chemical procedure were discovered to be unsatisfactory by the recovery test for the determination of niacin in feed additives containing much interfering substances. But the possibility of the determination of niacin in feed additives on chemical procedure together with microbioassay was proved.
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